Genetic Species Identification Using ycf1b, rbcL, and trnH-psbA in the Genus Pinus as a Complementary Method for Anatomical Wood Species Identification

This study proposes the use of genetic analysis as a complementary method for species identification in genus Pinus, particularly cases where anatomical is challenging. Pinus were grouped based on similarities, and efficacy using ycf1b, which most variable identification, rbcL, suggested DNA barcode land plants, was evaluated within each group. Sequences obtained from National Center Biotechnology Information database used to perform phylogenetic analysis. Among Group 1 (P. echinata, P. elliottii, ponderosa, radiata, rigida, taeda, virginiana), rbcL only effective identifying radiata while ycf1b classified five species. An additional barcode, trnH-psbA, needed identify taeda. In 2 densiflora, sylvestris, thunbergii), identified both with exception possible hybrids densiflora sylvestris. 3 koraiensis strobus), two ycf1b. Combining can accurately Pinus.